Fatameh Karimpour - ‎Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur ‎University of Medical Sciences, Ahvaz, Iran
Ghorban Mohammadzadeh - ‎‎Associate Professor, ‎Hyperlipidemia Research Center AND Department of Clinical Biochemistry, School of ‎Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Alireza Kheirollah - ‎‎Assistant Professor, ‎Cellular and Molecular Research Center AND Department of Clinical Biochemistry, ‎School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran‎
Mohammad Ali Ghaffari - ‎‎Professor, Cellular and Molecular Research Center AND Department of Clinical Biochemistry, ‎School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran‎
Azadeh Saki - Assistant Professor, Department of Biostatistics and Epidemiology, School of Public Health, Mashhad University of Medical Sciences, ‎Mashhad, Iran‎

BACKGROUND: Colesteryl ester transfer protein (CETP) plays a key role in the metabolism of lipoproteins; therefore, polymorphisms of its gene can affect susceptibility to coronary artery disease (CAD) in diabetes mellitus. The aim of the present study was to investigate association between I۴۰۵V polymorphism of CETP gene and risk of CAD in patients with type ۲ diabetes mellitus. METHODS: The current case-control study was conducted on ۱۴۳ patients with type ۲ diabetes and angiographically diagnosed CAD and ۱۵۰ patients with type ۲ diabetes and without CAD. Genotyping was performed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The presence of CAD was defined as higher than ۵۰% reduction in coronary artery diameter. RESULTS: The genotype frequencies of I۴۰۵V polymorphism were II (۲۷.۳% vs. ۲۳.۲%), IV (۶۱.۵% vs. ۶۷.۵%), and VV (۱۱.۲% vs. ۹.۳%) in diabetic with CAD compared to diabetic without CAD (χ۲ = ۱.۱۶۴) (P = ۰.۵۵). The I and V alleles were found at frequencies of ۶۳.۶% and ۶۱.۶% in the diabetic with CAD group and ۳۶.۴% and ۳۸.۴% in the diabetic without CAD group (χ۲ = ۰.۲۶۳) (P = ۰.۶۰). No significant difference was observed between two groups in terms of genotype and allele frequency. Moreover, no significant association was observed between II, IV, and VV genotypes and lipid profiles in both groups. However, a significant difference was observed between genotype distributions of I۴۰۵V polymorphism in men according to the severity of CAD. CONCLUSION: It is speculated that I۴۰۵V polymorphism may be associated with the severity of coronary artery stenosis only in men with type ۲ diabetes mellitus.

Cholesterol Ester Transfer Protein, Polymorphism, Type ۲ Diabetes Mellitus, Coronary Artery Disease