Fabrication, Characterization and BiologicalAssessment of Angiogenic Sponge Wound DressingContaining Advanced Platelet-Rich-Fibrin

Publish Year:

1401

نوع سند:

مقاله کنفرانسی

زبان:

English

View:

22

This Paper With 15 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

SETBCONF02_008

تاریخ نمایه سازی: 21 شهریور 1401

Abstract:

Hydrogel wound dressings with good biocompatibility and swelling capability are increasingly used in skin tissue engineering applications. Platelet concentrates such as platelet-rich fibrin (PRF) have also received a great deal of attention in recent years for use in wound treatments due to release of different types of growth factors. The composition of PRF in a hydrogel matrix for use as a wound dressing is a less studied topic. In this study, sponge hydrogel dressings were prepared from chitosan/polyethylene glycol (CsPEG) with different percentages (۱, ۱.۵ and ۲% w/v) of advanced PRF (A-PRF). Based on results, CsPEG sponge contains ۱.۵% w/v of A-PRF (CsPEG/۱.۵A-PRF) had the highest mechanical properties (tensile strength, elastic modulus and elongation at break) among all sponges and was therefore selected as the optimal sample for other tests. CsPEG/۱.۵A-PRF sponge also showed the most intense antibacterial activity against E. coli and S. aureus compared with other sponges. Enzyme-linked immunosorbent assays revealed that PDGF-AB and VEGF had an incrementally release from CsPEG/۱.۵A-PRF sponge in ۷ days. The results of MTT assay also showed that none of the prepared sponge dressings were cytotoxic and CsPEG/۱.۵A-PRF sample significantly increased L۹۲۹ cells proliferation and viability in ۵ days compared to other samples. Eventually, the results of this study introduce CsPEG/۱.۵A-PRF sponge as a suitable candidate for skin tissue engineering applications.

Authors

SaeidehSalehi
Saeideh Salehi

Advanced Materials Research Center, Department of Materials Engineering, NajafabadBranch, Islamic Azad University, Najafabad, Iran

NafiseKazemi
Nafise Kazemi

Advanced Materials Research Center, Department of Materials Engineering, NajafabadBranch, Islamic Azad University, Najafabad, Iran

MarjanMirhaj
Marjan Mirhaj

Department of Materials Engineering, Isfahan University of Technology, Isfahan ۸۴۱۵۶-۸۳۱۱۱, Iran

MohamadrezaTavakoli
Mohamadreza Tavakoli

Department of Materials Engineering, Isfahan University of Technology, Isfahan ۸۴۱۵۶-۸۳۱۱۱, Iran

JalehVarshosaz
Jaleh Varshosaz

Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery SystemsResearch Centre, Isfahan University of Medical Sciences, Isfahan, Iran

SheydaLabbaf
Sheyda Labbaf

Department of Materials Engineering, Isfahan University of Technology, Isfahan ۸۴۱۵۶-۸۳۱۱۱, Iran