Sara Parvin - Department of Microbiology, faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Farzaneh Broumand Sorkhabi - Department of Obstetrics and Gynecology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Elnaz Ohadi - Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Lida Lotfollahi - Department of Microbiology, faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

Backgrounds: Listeria monocytogenes is an opportunistic pathogen causing listeriosis, its pathogenicity is due to the presence of virulence factors including InlA, InlB, PlcA, PlcB, ActA, Iap, and Hly. The purpose of this study was to evaluate the formation of biofilm and its association with serotypes and virulence factors in L. monocytogenes isolates. Material and Methods: In this study, ۵۱ L. monocytogenes isolates were collected from blood, urine, feces, placenta, rectum, and vagina samples as well as livestock and food samples. Biofilm production was measured using microtiter plate assay, and virulence genes were identified by PCR method. Findings: Out of ۵۱ isolates, ۲۷ (۵۲.۹%) were non-biofilm producers, ۱۷ (۳۳.۳%) were weak biofilm producers, four (۷.۸%) were medium biofilm producers, and three (۵.۹%) were strong biofilm producers. According to this study results, different L. monocytogenes strains could form biofilm with various intensities. The actA, flaA, inlJ, inlA, and plcB genes were observed in all the isolates. The frequency of the hlyA, plcA, iap, inlB, and inlC genes among the isolates was ۹۰.۲, ۹۴.۱, ۹۸, ۸۸.۲, and ۸۲.۴%, respectively. There was no significant correlation between the presence/absence of virulence genes in biofilm producing and non-biofilm forming isolates, except for the inlC and iap genes, which showed a significant correlation with the ability to form biofilm. Conclusion: Due to the high prevalence rate of biofilm formation among the isolates and the importance of biofilm production in medical surfaces and food industries, eradication of biofilm-forming isolates is important.  

Biofilms, Serogroup, Listeria monocytogenes, Virulence factors