Ali Dehnoee - Faculty of Chemistry, Kharazmi University, Tehran, Iran.
Roozbeh Javad Kalbasi - Faculty of Chemistry, Kharazmi University, Tehran, IRAN.
Mohammad Mahdi Zangeneh - Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Mohammad-Reza Delnavazi - Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Akram Zangeneh - Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.

The present study investigates the biosynthesis of Ag nanostructures (AgNPs) via the mediation ofthe methanolic extract of Heracleum persicum seeds, without utilizing any stabilizer or surfactant. Thesenanostructures were identified utilizing ultraviolet-visible spectroscopy (UV-Vis), field emission scanningelectron microscopy, energy-dispersive X-ray spectroscopy (FESEM-EDX), X-ray diffraction (XRD), Highresolutiontransmission electron microscopy (HR-TEM), Fourier-transform infrared spectroscopy (FTIR) andDynamic light scattering (DLS). The attributes of AgNPs versus usual human breast adenocarcinoma cell linesi.e. Hs ۲۸۱.T, MDA-MB-۴۶۸, AU۵۶۵ [AU-۵۶۵], MCF۷, CAMA-۱, SK-BR-۳, NMU, and RBA were evaluated.The livability of breast adenocarcinoma cell line diminishes dose-dependently in the existence of AgNPs.Subsequent to clinical survey, AgNPs can be applied as a green drug with inside the remedy of human breastadenocarcinoma.

Breast adenocarcinoma; Plant extract; Cell viability; MTT assay; Silver nanostructures