Sathiya D - PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore – ۶۴۱ ۰۱۸, Tamil Nadu, India
P Viswanathan - PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore – ۶۴۱ ۰۱۸, Tamil Nadu, India
K Kalimuthu - PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore – ۶۴۱ ۰۱۸, Tamil Nadu, India
A Vanitha - PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore – ۶۴۱ ۰۱۸, Tamil Nadu, India

The development of biologically enthused green synthesis of silver nanoparticles (SNPs) has concerned significant global awareness about medical science and disease treatment. This paper discusses the green synthesis of SNPs using organic green sources; here we report a facile bottom-up ‘green’ route for the synthesis of SNPs using aqueous leaves extract of Pogostemon speciosus (Benth.) and evaluate its in-vitro anti-inflammatory, antibacterial and photocatalytic activities. The nanoparticles were investigated for the preparation of denaturation particles with PSLASNPs and the evaluation of anti-inflammatory activity with Protein denaturation and HRBC stabilization assays. Later, these PSLASNPs were studied for their potential role in antibacterial activity by well diffusion method, and Photocatalytic activity on degradation of dyes was demonstrated by using dyes Crystal violet, Coomassie blue, and Congo red. At ۱۰۰۰ µg/ml, the PSLASNPs have the greatest prevention of protein denaturation (۷۱.۹۲±۱.۳۷%), whilst the stabilization of the HRBC membrane exhibited significant anti-inflammatory action (۶۴.۳۹±۱.۶۱ %). The PSLASNPs showed the best antibacterial activity at the concentration of ۱۰ µg/ml against Bacillus subtilis (۸.۲ mm), followed by Pseudomonas stuberia (۶.۲ mm) and Escherichia coli (۶.۴ mm), Staphylococcus aureus (۵.۳ mm), Staphylococcus gallinarium (۴.۵ mm) respectively at the same concentrations. Crystal violet, Coomassie blue, and Congo red were used for Photocatalytic activity on the breakdown of dyes. After ۳۵ minutes, the degradation process was determined to be complete by the transformation of the reaction mixture's color to colorless. As a result, the PSLASNPs have anti-inflammatory, antibacterial, and photocatalytic activities.

Green synthesis, Protein denaturation assay, HRBC stabilization assay, Antibacterial activity, Dye degradation