A bioinformatics analysis of Runx۱ and its related miRNA Involved in Colorectal Cancer metastases

Backgrounds: Colorectal cancer is one of the most frequent carcinoma worldwide, and it is alsoone of the most important causes of death in the world. MicroRNAs (miRNAs or miRs) aresmall single-stranded non-coding RNA molecules that play key roles as master regulators ofmany biological processes altered in cancers such as colorectal cancers. Additionally, they canbe used to diagnose, treat and monitor cancer. Here, we aimed to examine the crosslink betweensome of these miRNAs and a specific gene in colorectal cancer. The association between theexpression of genes and miRNAs is significant, and changes in their expression levels can onsetcancer.Materials and Methods: Runt-related transcription factor ۱ (RUNX۱) acts as an oncogene intumors. Abnormal upregulation of RUNX۱ has been suggested to contribute to thecarcinogenesis of colorectal cancer. By using the miRTargetLink database, we found out thatRUNX۱ is strongly associated with certain miRNAs. Finally, using the Diana miRpath V.۲algorithm, we found out that it has a significant relationship with miR-۱۰۶a-۵p, miR-۲۰a-۵p, andmiR-۱۷-۵p.Results: Data analysis showed that miR-۱۰۶a-۵p, miR-۲۰a-۵p, and miR-۱۷-۵p weresignificantly associated with the Runx۱ gene, which could be associated with the TGF-beta, p۵۳,and foxO signaling pathways. Due to the effect of the TGF-β signaling pathway on cellmigration, this can affect cancer metastasis.Conclusion: These results revealed RUNX۱ could regulate colorectal cancer migration via theTGF-β signaling pathway, and RUNX۱ might serve as a potential target for preventing colorectalcancer metastasis.

Keywords:

TGF-beta , colorectal cancer , miRNA , Runx۱