Ki-۶۷ marker and neurotrophic genes expression in metformin-treated mesenchymal stem cells in ۴۸ hours

Backgrounds: Metformin is the foremost common prescribed drug for type ۲ diabetes. There isincreasing evidence that metformin can be used in a variety of therapeutic conditions due to itsbiological effects. The Ki-۶۷ antigen is expressed in all proliferating cells (normal and tumorcells), so this marker is used to determine the rate of cell population growth. Neurotrophicfactors are large polypeptides that contribute to development and survival of the central andperipheral nervous systems.Materials and Methods: In this study bone marrow cells were isolated from the femora andtibia bones, after the third passage the extracted stem cells were cultured in a ۹۶-well plate andtreated with ۱, ۵, ۱۰, ۱۵, and ۵۰ μM of metformin for ۴۸ h. The proliferation rate of cells andgenes expression was assessed by Ki-۶۷ marker and RT-PCR method.Results: The results of this study indicate that the proliferation rate in the ۱۰ μM of metformingroup showed a significant increase compared to control groups. Analysis of gene expressionshowed that the expression of BDNF and GDNF genes in ۵۰ μM and NT۳ in ۱۰ μM had thehighest expression.Conclusion: Metformin may be suggested as a pre-therapist to strengthen mesenchymal stemcells before transplantation for the treatment of neurodegenerative diseases and seems anappropriate inducer factor to induce neurotrophic genes and can be used to treatneurodegenerative diseases like Alzheimer’s.

Keywords:

Ki-۶۷ marker , Mesenchymal stem cells , Metformin , Neurotrophic genes