Kaveh Hatami Kahkesh - Department of Basic Medical Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
Ahmad Mahmoudi - Department of Animal Science, Islamic Azad University, Esfahan, Iran
Amin Ahmadi - Faculty of Veterinary Medicine, Ardakan University, Ardakan, Iran
Melika Ghobadi - Division of Genetics, Department of Biology, Nourdanesh Institute of Higher Education, Meymeh, Iran
Kamran Hatami - Department of Biotechnology, Ramin University of Agriculture and Natural Resources, Ahwaz, Iran
Mohammad Taha Salmanifard - Department of biology, Department of Biotechnology, Yazd University, Yazd, Iran

Background: Liver cancer (LC) is one of the most invasive malignancies and may derive fromdifferent types of liver cells with an estimated more than ۷۸۱۰۰۰ deaths in ۲۰۱۸. The Rho-familyGTPase Rac۱ plays a key role in carcinogenesis and inflammatory responses. Epidemiologicalstudies demonstrated a relation between chronic inflammation and cancer. Most cases ofhepatocellular carcinoma (approximately ۸۰ %) are associated with cirrhosis related to chronichepatitis following viral infections.Materials and Methods: Based on bioinformatics analysis, hsa-mir-۱۸۲-۵p was selected. Usingby Mirwalk database, Prediction data of genes was collected. Then in the NCBI database, atUniGene, we have checked genes expression. Functional annotation analysis has been parsedwith David database. Pathways relations were collected at KEGG in David database.Results: ۴۱۵ genes were identified for LC. Information of gene expression shows that Rac۱genes were down-expression in normal livers. In the other hand, hsa-miR-۱۸۲-۵p had upexpression.Conclusion: These results suggest that Rac۱ may be a new gene therapy target for LC. BlockingRac۱ expression in LC cells induces apoptosis of these tumor cells and may thus represent a newtherapeutic approach.

Liver Cancer, microRNA, RAC۱, has-miR-۱۸۲