Application of gene expression sequencing in COVID-۱۹ pandemic diagnostics

Background: In late December ۲۰۱۹, an outbreak of an unknown disease called coronavirusoccurred in Wuhan, Hubei Province, China, leading to a sharp increase in global mortality.Therefore, the need for early prevention through the accurate and timely diagnosis of the diseaseis needed to control and prevent its recurrence as a powerful and cost-effective tool for highpowertranscription analysis.Materials and Methods: In this study, applied diagnostic methods were proposed, which, basedon research by next-generation sequencing technology (NGS), provided a powerful tool forstudying emerging pathogens/diseases, including whole-genome sequencing, RNA-Seq, andsingle-cell sequencing. Gene expression pattern analysis and pathogen detection are used. In thisstudy, the RNA-Seq platform for rapid detection of the COVID-۱۹ epidemic was examined bythe Galaxy instrument.Results: Traditional approaches challenge the detection of complex and massive interactionsbetween viruses and host/cell. There are many diagnostic methods, but the main advantage ofNGS is that it can detect viral agents without prior notice and provides reports from laboratoriesaround the world for high-volume COVID-۱۹ tests.Conclusion: With this tool, cells containing active viruses can be identified, several viruses canbe combined in one cell, and expression can be distinguished between infected and non-infectedcells. This helps us understand how viruses play a role in stimulating or modifying diseasedevelopment in specific diseases and developing new therapies to target viruses. Identifying awide range of pathogens using NSG technologies is also essential in controlling viral infectionscaused by a new pathogen.

Keywords:

Covid۱۹ , Diagnosis , RNA-Seq , Gene expression , NGS sequencing