Bioinformatics analysis Identified miR-۴۸۷a-۳p in human dilated cardiomyopathy

Backgrounds: Dilated cardiomyopathy (DCM) is a common type of non‑ischemiccardiomyopathy, which causes heart failure and sudden cardiac death worldwide. MicroRNAs(miRNAs) are small noncoding RNAs that play crucial roles in post-transcriptionally regulatinggene expression, by targeting ۳'UTR of their target mRNAs. Recently, they are introduced aspromising biomarkers in cardiovascular disease. The present study aimed to predict andcandidate circulating miRNAs, as potential biomarkers for DCM.Materials and Methods: Data from the National Center for Biotechnology Information GeneExpression Omnibus were employed to investigate the differential expression of miRNAs in aserum of DCM patients versus normal ones. Then, the gene targets of miRNAs were determinedthrough the miRWalk database. Consequently, the GTEx portal database was used to attain theexpression of gene in tissues.Results: Our bioinformatics analysis demonstrated that hsa-miR-۴۸۷a-۳p is differentiallyexpressed in serum samples of DCM patients. According to miRWalk data, this miRNA couldpossibly target ۳'UTR of MYPN and TPM۱ which have high expression in heart tissues as well asmutation in DCM.Conclusion: Altogether, our analysis suggests that there is correlation between hsa-miR-۴۸۷a-۳p, MYPN and TPM۱ genes expression and DCM. With performing functional analysis alongwith bioinformatics analysis, this miR-۴۸۷a-۳p appeared as a potential diagnostic biomarker forDCM.

Keywords:

Dilated cardiomyopathy , miR-۴۸۷a-۳p , MYPN , TPM۱