Effect of Cytokines Gene Expression and Serum Level of Vitamin D on the Severity of COVID-۱۹

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نوع سند: مقاله ژورنالی
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JR_IJMM-16-5_007

تاریخ نمایه سازی: 16 مهر 1401

Abstract:

Background and Aim: The COVID-۱۹ disease is an emerging infectious disease that appeared in December ۲۰۱۹ in Wuhan, China. An uncontrolled systemic inflammatory response is one of the primary mechanisms causing death in this disease. In this study, the expression levels of some inflammatory cytokines, vitamin D, and some hematological and biochemical parameters were compared in patients with severe COVID-۱۹ and mild types. Materials and Methods: In this cross-sectional study, ۶۰ blood samples were taken from ۳۰ severe coronavirus patients and ۳۰ mild coronavirus patients. The expression levels of cytokines such as IL (interleukin)-۶, interferon (IFN)-α, IL-۱۲, transforming growth factor (TGF) β, IL-۸ and tumor necrosis factor (TNF)-α were evaluated using Real-time PCR. A T-test was used for Statistical Analysis. Results: IL-۶, IFN-α, IL-۱۲, TGF-β, IL-۸, and TNF-α cytokines in the peripheral blood of severe patients, were positive in ۲۸/۳۰ (۹۳.۳۳%), ۲۷/۳۰ (۹۰%), ۲۴/۳۰ (۸۰%), ۲۵/۳۰ (۸۳.۳۳%), ۲۶/۳۰ (۸۶.۶۶%), and ۲۷/۳۰ (۹۰%) respectively. The positive rate of these cytokines in the mild patients were ۲۰/۳۰ (۶۶.۶۷%), ۲۱/۳۰ (۷۰%), ۱۸/۳۰ (۶۰%), ۱۷/۳۰ (۵۶.۶۷%), ۱۹/۳۰ (۶۳.۳۳%), ۱۸/۳۰ (۶۰%), respectively. There was a statistically significant difference between these two groups in terms of cytokines biomarkers. A significant difference was found between both groups in terms of the serum level of lactate dehydrogenase (LDH), the mean number of lymphocytes and neutrophils as well as the mean percentage of neutrophils/ lymphocytes ratio (NLR). Conclusion: The expression of cytokine genes and their release into the peripheral blood was increased in both severe and mild patients with COVID-۱۹. However, they were more intense in patients with severe symptoms than those with mild symptoms and can cause inflammatory and even destructive reactions. Vitamin D deficiency plays no role in causing severe COVID-۱۹ in patients without risk factors. Severe COVID-۱۹ is characterized by elevated serum levels of LDH and NLR≥۳.۴۵.

Authors

Mehdi Kazempour Dizaji

Biostatistics Department, Mycobacteriology Research Center, National Research Institute of Tuberculosis and lung Diseases, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Hamidreza Jamaati

Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

Naghmeh Bahrami

Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Behrooz Farzanegan

Critical Care Quality Improvement Research Center at Shahid Modarres Hospital, Department of Anesthesiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mahsa Rekabi

Pediatric Respiratory Diseases Research Center, National Research Institute of Tuberculosis & Lung Diseases (NRITLD), Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mogtaba Mokhber Dezfuli

Lung transplantation Research Center, National Research Institute of Tuberculosis, and lung Diseases, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Jalal Heshmat Nia

Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mohammadreza Madani

Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mahya Daustani

Department of Biotechnology, Faculty of Sciences, Islamic Azad University, Tehran, Iran

Sadegh Shirian

Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Ladan Masoumi

Shefa Khatam Neuroscience Research Center, Tehran, Iran

Amir Ghaemi

Department of Virology, Pasture Institute, Tehran, Iran

Armita Narimani

Department of Cell and Molecular Biology, School of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran

Mehran Khakbaz

Department of Cell and Molecular Biology, School of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran

Abdolreza Mohamadnia

Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mohammad Varahram

Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

Ali Akbar Velayati

Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

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