زهرا پشنگه - hormozgan bandarabbas
منصوره شمیلی - Associate professor, Department of Horticulture, Faculty of agriculture, University of Hormozgan
نگار نگارآذرپیرا - Shiraz University of Medical Sciences
علیرضا یاوری - hormozgan bandarabbas

Guava (Psidium guajava L.), is adapted to an extensive range of soils, however, its development is strongly affected by salinity. Environmental stresses are of the major and influential factors in the amount of secondary metabolites, especially phenolic compounds present in plants. The purpose of this investigation was to study the leaf antioxidant and flavonoid contents (catechin, quercetin and rutin) of guava seedlings grown under normal growth conditions or exposed to ۱۰۰ mM salinity through irrigation water. Leaf samples collected ۱۲ weeks after salinity stress treatment and total phenol, antioxidant capacity, malondialdehyde, catalase, peroxidase and protease activities and high performance liquid chromatography were examined.  According to the results, salinity enhanced the amount of total phenol from ۱۵.۳۱ mgg-۱ GA (control) to ۳۲.۱۲ mgg-۱ GA. The MDA value improved in guava leaves from ۰.۴۲ mg g-۱FW under non-stress conditions to ۰.۷۶ mgg۱ FW under salinity. The most catalase (۱۸۲.۱۱ μmol min-۱g-۱FW), protease (۹.۷۰ μmol min-۱g-۱ FW) and peroxidase (۱۹.۹۹ μmol min-۱g-۱ FW) activities were observed in salt exposed plants. The antioxidant capacity of leaf extract was ۶۲.۹۰% in control plants, which enhanced to ۷۷.۴۶% after salt treatment. The most abundant flavonoid composition of leaf extract was rutin (۷۷۰ μg mg-۱DW) in the control treatment and catechin (۱۱۱۰ μg mg-۱DW) in salt exposed plants. We concluded salinity stress, in addition to a noticeable increase in the activities of catalase, peroxidase and protease enzymes; enhanced phenol, catechin and quercetin contents.  

Antioxidant capacity, Catalase, Catechin, Peroxidase, Total phenol, Malondialdehyde, پراکسیداز, ظرفیت آنتی اکسیدانی, فنل کل, کاتالاز, کاتچین, مالون دی آلدئید