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Investigation of phylogenetic relationships and genetic diversity of population (Seidlitzia rosmarinus) by RAPD marker in some regions of Iran

عنوان مقاله: Investigation of phylogenetic relationships and genetic diversity of population (Seidlitzia rosmarinus) by RAPD marker in some regions of Iran
شناسه ملی مقاله: JR_ROST-23-1_006
منتشر شده در در سال 1401
مشخصات نویسندگان مقاله:

شیما صدیقی - PhD Student, Science and Research Branch, Islamic Azad University, Tehran, Iran
علیرضا ایرانبخش - Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
سید محمد مهدی حمدی - Associate Prof., Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
ایرج مهرگان - Associate Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

خلاصه مقاله:
The genus Sedlitzia (Amaranthaceae) includes four herbaceous and one shrubby species. Sedlitzia rosmarinus is resistant to salinity that plays an important role in soil conservation. In this research, RAPD molecular markers were used to evaluate the genetic diversity among ۸۵ plant samples from ۱۷ populations of S. rosmarinus. Five primers were used to perform RAPD reactions and a total of ۴۰۰ DNA bands were obtained. After forming ۰ and ۱ matrix with the help of GenAlex ۶.۴۱ and UPGMA methods using SHAN clustering model, NTSYS Ver. ۲.۰۲ software was used to interpret RAPD analysis data. Molecular analysis of variance (AMOVA) was performed to estimate genetic diversity out of ۸۵ individuals. Out of the total diversity, it was calculated that, the highest amount of genetic diversity i.e. ۶۹% was related to intra-population and ۳۱% to inter-population diversity, respectively.  

کلمات کلیدی:
Amaranthaceae, AMOVA, cluster analysis, Salinity, UPGMA method

صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1537127/