زینب حیدریان - MSc graduated, Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
محمد جوان نیکخواه - Prof., Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran (jnikkhah@ut.ac.ir)
خلیل بردی فتوحی فر - Assistant Prof., Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
عبداله احمدپور - PhD Student, Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

The Gibberella fujikuroi species complex with Fusarium anamorphs is the causal of diverse diseases of many important plants. Many of the known species in this species complex produce a wide range of secondary metabolites. In this study, morphological characteristics of ۴۶ isolates from rice, maize, sugarcane and onion and phylogenetic relationships of ۲۲ isolates on the basis of ITS۱-۵.۸S-ITS۲ region and EF-۱α gene sequences were investigated. According to the morphological characteristics of ۴۶ isolates, five species including F. fujikuroi, F. proliferatum, F. sacchari, F. thapsinum, and F. verticillioides were identified. Moreover, earlier morphologically two isolates obtained from sugarcane were very similar to F. verticillioides but, using EF-۱α gene sequence were identified as F. andiyazi. On the basis of EF-۱α sequence, six clades were identified and differentiated all of six species (F. andiyazi, F. fujikuroi, F. proliferatum, F. sacchari, F. thapsinum and F. verticillioides) from one another. Moreover, sequencing of ITS۱-۵.۸S-ITS۲ region identified five clades and differentiated F. proliferatum and F. verticillioides from each other but this region could not differentiate F. proliferatum and F. fujikuroi or F. andiyazi and F. verticillioides from each other. Generally, EF-۱α gene was appropriate for differentiation of Gibberella fujikuroi species complex.