Mina Fartousi - Department of Genetics, Faculty of Biological Sciences, Islamic Azad University ,Varamin -Pishva Branch, Varamin, Iran
Jamil Zargan - Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran
Shohreh Zare Karizi - Department of Genetics, Faculty of Biological Sciences, Islamic Azad University ,Varamin -Pishva Branch, Varamin, Iran
Sajedeh Zargan - Department of Medical Radiation Engineering, Islamic Azad University, Central Tehran Branch, Tehran, Iran
Ashkan Haji Noor Mohammadi - Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran
Mohsen Mousavi - Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran
Hani Keshavarz Alikhani - Department of biology, Faculty of science, Razi University, Kermanshah, Iran

Objectives: Breast cancer is one of the most common types of cancer among women. One of these toxins that inhibits the growth of breast cancer cells in ۲D cell culture and has significant anti-tumor effects is Dianthin-۳۰. Therefore, in this manuscript, for the first time, the anti-cancer effect of Dianthin-۳۰ toxin against breast cancer cells (MCF-۷) in ۳D culture has been studied. Materials and Methods: In order to evaluate the anti-cancer effects and cytotoxicity of the toxin at concentrations of ۱.۲۵, ۲.۵, ۵ and ۱۰μg/ml, MTT methods were used and a Neutral red test was used to validate the results of this test. Nitric oxide, Catalase, GSH assays, cytochrome c, Caspase-۳ and Comet assay tests were also used to determine the type of mortality in cancer cells. Results: This toxin did not induce nitric oxide production, but at concentrations higher than ۵μg/ml increased catalase production compared to the control. However, the level of GSH produced in all of the concentrations was significant compared to the control. In addition, Dianthin-۳۰ increased cytochrome ۳۰ and activation of caspase-۳ in the above concentrations, but this effect was not significant compared to the control. The results of alkaline comet test also showed that the rate of induction of apoptosis by toxin was upward compared to the control. Conclusion: The results of this study show that Dianthin-۳۰ has anti-cancer effects and has caused death in breast cancer cells and this toxin probably induced apoptosis in cancer cells more than the non-mitochondrial pathway.  

Breast cancer, Dianthins ۳۰, Cytotoxicity, MCF-۷ cell line, ۳D culture