Histopathologic evaluation of the inflammatory factors and stromal cells in the endometriosis lesions: A case-control study
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJRM-20-10_003
تاریخ نمایه سازی: 17 آبان 1401
Abstract:
Background: Endometriosis is a multifaceted gynecological disorder defined as a benign estrogen-dependent chronic inflammatory process in which endometrial glands and stroma-like tissues are located outside the uterine cavity. It affects around ۲-۱۰% of all women during their reproductive years.
Objective: This study aimed to evaluate the traffic of mesenchymal stem cells and inflammatory factors toward the lesions.
Materials and Methods: ۱۰ samples of normal endometrium and eutopic endometrium were studied as a control group and ۱۰ ectopic samples were considered as a case group. Hematoxylin and eosin staining was used to evaluate stromal cells and inflammatory cells. Immunohistochemical staining was performed to show the presence of proliferating cell nuclear antigen in the lesions. The cells were digested and cultured in the laboratory to study cell proliferation. The number of cells and vessels were counted with Image J software, and data analysis was performed with Prism software.
Results: Data analysis showed that the number of stromal cells and vessels in ectopic tissue were significantly higher than the control group (p < ۰.۰۰۱). Also, the number of inflammatory cells, including neutrophils, monocytes, lymphocytes, and macrophages, in the ectopic group was much higher than in the control group (p < ۰.۰۰۵).
Conclusion: By expanding the number of blood vessels, blood flow increases, and cell migration to tissues is facilitated. The accumulation of inflammatory cells, especially macrophages, stimulates the growth of stem cells and helps implant cells by creating an inflammatory process.
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Authors
Ali Mohebbi
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Vida Hojati
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Masoumeh Majidi Zolbin
Pediatric Urology and Regenerative Medicine Research Center, Gene, Cell, and Tissue Research Institute, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Reza Aflatoonian
Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
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