Investigation of Biofilm Formation and Antibiotic Resistant of Bacteria Isolated from Septic Neonates

Neonatal sepsis refers to the bacterial bloodstream infections of the newborn during the neonatal period as usually the first twenty-eight days of life. The current study was done in the laboratories of AL-Batool Teaching Hospital for Gynecology and Pediatrics in Baqubah, Diyala Governorate, including ۱۴۰ blood specimens collected from the neonates admitted to the hospital with suspected sepsis, the ages of the both groups was ranged from ۱ day to ۲۸ days. Out of the total cultured samples, ۳۲.۱۴% (۴۵ of ۱۴۰) were positive and ۶۷.۸۶% (۹۵ of ۱۴۰) were negative blood culture. ۴۵ of ۱۴۰ samples were negative to the blood culture chosen as control group. The results showed highest isolates were Coagulase Negative Staphylococcus (CoNS) ۱۹ (۴۲.۲%), followed by Staphylococcus aureus ۱۱ (۲۴.۵%), Escherichia coli ۴ (۸.۸%), Klebsiella pneumonia ۴ (۸.۸%), Acinetobacter baumannii ۳ (۶.۷%), Group B Streptococcus (GBS), and Pseudomonas aeruginosa ۲ (۴.۵%). Ceftazidime antibiotic has the highest resistance percentage followed by CIP, CXM, AMP, NA, C, CD, and CL among the studied bacterial isolates. Biofilm formation of isolates showed all bacterial isolates of K. pneumonia, A.baumannii, P.aeruginosa, and GBS  by ۱۰۰% can form biofilm, while the isolates of S.aureus, CoNS and E.coli were ۶ (۵۳.۵۵%), ۷ (۳۶.۸۴%), and ۲ (۵۰%) biofilm forming, respectively. These biofilm-forming isolates exhibited high resistance to AK, AMC, TM, CTX, NE, VAN, COT, CL, CAZ, AMP, NA, C, and CIP.

Keywords:

Sepsis , Biofilm formation , Antibiotics resistance