Isolation and characterization of Kaempferol from Tapinanthus globiferus growing on Balanites aegyptiaca

Tapinanthus globiferus is a hemi-parasitic plant used in ethonomedicine to treat different ailment including fungal infections. The aim of the study was to isolation the bioactive compound from leaf of T.globiferus growing on Balanites aegyptiaca. The plant material was collected, identified pulverized to powder using mortar and pestle. The powdered plant material was subjected to maceration with ۹۰% methanol to obtained crude methanol leaf extract which was further partitioned with solvent of increasing polarity to obtained n-hexane, chloroform, ethylacetate and n-butanol fractions. Chromatographic separation of ethylacetate fraction using a combination of silica gel column and sephadex LH-۲۰ column led to the isolation of flavonoid (۳,۵,۷- trihydroxy-۲-(۴-hydroxyphenyl)-۴H-chromen-۴-one). The structure of the compound was established by spectroscopic analysis (FTIR, NMR). To the best of our knowledge, this is the first report of isolation of this compound from the leaf of T. globiferus growing on Balanite aegyptiaca. [m۱]