Influence of plant growth regulators, carbohydrate source and concentration on micropropagation and other physiological traits of grape (Vitis vinifera L. cv. Shahroudi) under in vitro conditions

Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
View: 37

This Paper With 10 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_BREDNG-9-1_006

تاریخ نمایه سازی: 17 آذر 1401

Abstract:

The grapevine (Vitis vinifera L.) is a native of central Asia and has been propagated by various methods, including in vitro propagation. Present research was conducted to study the effect of plant growth regulators (BAP, IBA), carbon sources (sucrose, glucose, fructose, table sugar) and their concentrations (۳۰, ۶۰ and ۹۰ g/l) on the proliferation, root induction, protein, chlorophyll and carotenoid contents of the grape (Vitis vinifera L. cv. Shahroudi) in in vitro conditions based on a completely randomized design with three replications, using nodal explants. The result showed that the highest rate of proliferation (۳.۰۴ shoots per explant) occurred at MS medium, containing ۱.۵ mg/l BAP plus constant amounts of GA۳ (۰.۳ mg/l) and IBA (۰.۱ mg/l). The highest rate of root induction (۸۸.۸۸%) was obtained at ۱/۲ MS medium at ۰.۵ mg/l IBA. Type and concentration of carbon source had a significant effect on some of the measured characteristics. The highest plantlet height was obtained in MS medium supplemented with ۳۰ g/۱ table sugar and sucrose. Also, the highest number of shoots per explant (۳.۵ shoots) belonged to the use of ۳۰ g/l sucrose in the culture medium, followed by ۳۰ g/l table sugar (۳.۲۳ shoots). The highest value of chlorophyll a was observed for ۹۰ g/l glucose, followed by ۳۰ g/l sucrose and ۶۰ g/l table sugar. The highest chlorophyll b contentwas obtained for ۶۰ g/l table sugar, followed by ۳۰ g/l table sugar and sucrose. In general, with regard to most of the characteristics under study, it could be inferred that the propagation efficiency of ۳۰ g/۱ sucrose or table sugar was better than other carbon sources. Considering economic reasons and time, these treatments can be recommended for the commercial micropropagation of the Shahroudi cultivar of grapevine, instead of traditional methods of propagation.

Authors

علیرضا تاری نژاد

Department of Biotechnology, Faculty of Agriculture, Shahid Madani University, Tabriz, Iran

صالح امیری

Academic Staff Member of Agricultural Biotechnology Research Institute of Iran (ABRII), Northwest and West Region, Tabriz, Iran.

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Abido AIA, Aly MAM, Hassanen SA and Rayan GA, ۲۰۱۳. ...
  • Abou Rayya MS, Kassim NE and Ali EAM, ۲۰۱۱. Effect ...
  • Abraham E, Hourton-Cabassa C, Erdei L and Szabados L, ۲۰۱۰. ...
  • Alizadeh B and Tarinejad A, ۲۰۱۰. Application of MSTATC software ...
  • Comparative performance of in vitro multiplication in four grape (Vitis spp.) rootstock genotypes [مقاله ژورنالی]
  • Banilas G and Korkas E, ۲۰۰۷. Rapid micro-propagation of grapevine ...
  • Barreto MS and Nookaraju A, ۲۰۰۷. Effect of auxin types ...
  • Bradford MM, ۱۹۷۶. A rapid and sensitive method for the ...
  • Chee R, Pool RM and Bucher D, ۱۹۸۴. A method ...
  • Gautheret RJ, ۱۹۵۵. The nutrition of plant tissue cultures. Annual ...
  • Gopal J, Chamail A and Sarkar D, ۲۰۰۴. In vitro ...
  • Gray DJ and Benton CM, ۱۹۹۱. In vitro micropropagation and ...
  • Heloir M-C, Fournioux J-C, Oziol L and Bessis R, ۱۹۹۷. ...
  • Ilczuk A, Jagiełło-Kubiec K and Jacygrad E, ۲۰۱۳. The effect ...
  • Jaskani MJ, Abbas H, Sultana R, Khan MM, Qasim M ...
  • Kieber JJand Schaller GE, ۲۰۱۴. Cytokinins. Arabidopsis Book ۱۲: e۰۱۶۸. ...
  • Lee N and Wetzstein HY, ۱۹۹۰. In vitro propagation of ...
  • Lewandowski VT, ۱۹۹۱. Rooting and acclimatization of micropropagated Vitis labrusca ...
  • Li Y, He N, Hou J, Xu L, Liu C, ...
  • Lichtenthaler HK and Wellburn AR, ۱۹۸۳. Determination of total carotenoids ...
  • Mamiya K and Sakamoto Y, ۲۰۰۰. Effects of sugar concentration ...
  • Murashige T and Skoog F, ۱۹۶۲. A revised medium for ...
  • Singh SK, Khawale RN and Singh SP, ۲۰۰۴. Technique for ...
  • Swamy MK, Sudipta KM, Balasubramanya S and Anuradha M, ۲۰۱۰. ...
  • Talcott ST, Hernandez-Brenes C, Pires DM and Del Pozo-Insfran D, ...
  • Tarinejad A, ۲۰۱۳. Effects of disinfectants and antibiotics on contamination ...
  • Yaseen M, Ahmad T, Sablok G, Standardi A and Hafiz ...
  • نمایش کامل مراجع