Study the Effect of Swimming Training and Curcumin on Reduction of BAX and P۵۳ proteins and Increase in BCL۲ protein in Heart Tissue of Rats During Withdrawal of Excessive Ethanol Consumption

Background and objectives: Epidemiological studies have shown that high dose of ethanol can lead to apoptosis. On the other hand, the consumption of medicinal plants such as turmeric in the diet and performing physical activities are considered as factors to control apoptosis. Therefore, this article aims to mainly evaluate the anti-apoptotic effects of swimming and curcumin interactively during withdrawal of binge ethanol exposure in heart tissues. Material and Methods: In this study, ۴۰ rats were received ethanol (۲۵% W/V) as gavage for four days every eight hours. Then, ۷ days after ethanol withdrawal, they were classified in ۵ groups, including: (۱) control, (۲) curcumin, (۳) swimming, (۴) swimming with curcumin, and (۵) sham. Groups ۳ and ۴ performed swimming five sessions a week for two weeks, and groups ۲ and ۴ received ۵۰ mg/kg of curcumin five times a week for two weeks. The amount of BAX, BCL۲, and P۵۳ protein and BCL۲/BAX ratio were measured using western blot technique. The data were tested using the independent t-test at a significant level (p≥۰.۰۵) and two-way ANOVA. Results: Swimming had a significant effect on reduction of p۵۳ (F=۶۰.۰۵۱, P<۰.۰۰۰۱, η۲=۰.۷۴۱), Bax (F=۶۲.۵۹۴, P<۰.۰۰۰۱, η۲=۰.۸۸۷) and Bax/Bcl-۲ ratio (F=۲۹۰.۵۹۱, P<۰.۰۰۰۱, η۲=۰.۹۷۳), and increase of Bcl-۲ (F=۱۵۰.۹۴۰, P<۰.۰۰۰۱, η۲=۰.۹۵۰); Curcumin had a significant effect on decrease of p۵۳ (F=۵.۵۱۳, P=۰.۰۲۹, η۲=۰.۲۰۸), Bax (F=۶۶.۱۴۶, P<۰.۰۰۰۱, η۲=۰.۸۹۲) and Bax/Bcl-۲ ratio (F=۲۶۰.۶۵۵, P<۰.۰۰۰۱, η۲=۰.۹۷۰), and increase of Bcl-۲ (F=۷۳.۲۷۴, P<۰.۰۰۰۱, η۲=۰.۹۰۲). The results from two-way ANOVA showed the interactive effect of exercise and curcumin on the decrease of BAX (F=۳۵.۸۴۷, P<۰.۰۰۰۱, η۲=۰.۸۱۸) and BAX/BCL-۲ ratio (F=۱۷۵.۸۸۷, P<۰.۰۰۰۱, η۲=۰.۹۵۶), and the increase of BCL-۲ (F=۲۱.۲۰۵, P=۰.۰۰۲, η۲=۰.۷۲۶) were significant; but it had no significant effect on p۵۳ (F=۰.۰۰۰, P=۰.۹۹۹, η۲=۰.۰۰۰). Conclusion: Swimming and curcumin consumption simultaneously can significantly moderate apoptosis caused by ethanol in the heart tissue.

Keywords:

Curcumin , Ethanol , Apoptosis