Ahmad Alinaghi Langari - Student of Medicine, Student Research Committee, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Nazanin Sabet - Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
Hamideh Bashiri - Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
Sobhan Mohammadi Jorjafki - Student of Medicine, Student Research Committee, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Reyhaneh Rezaie - Student of Medicine, Student Research Committee, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Nafise Esmaeilpour - Student of Medicine, Student Research Committee, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Sedigheh Amiresmaili - Bam University of Medical Sciences, Bam, Ker.man, Iran
Mohammad Khaksari - Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran & Department of Physiology and Pharmacology, Afzalipour School of Medicine, Kerman University
Shahryar Dabiri - Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran
Mahmoud Amiri - Student of Medicine, Student Research Committee, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Zahra Soltani - Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran & Department of Physiology and Pharmacology, Afzalipour School of Medicine, Kerman University

Background: In our previous studies, the effect of sex hormones on brain edema reduction after traumatic brain injury (TBI) was demonstrated. In the current study, alone and combined effects of ۱۷-β estradiol (E۲) and vitamin D (Vit D) on TBI in male rats were investigated.Methods: Male rats were divided into six groups, including sham, TBI, vehicle, E۲, Vit D, and E۲+Vit D. In all groups except sham, moderate-intensity diffuse TBI was induced by the Marmarou’s method. Vehicle, E۲, Vit D and their combination were intramusculary injected one and ۱۲ hours after the TBI. The brain water content, permeability of blood brain barrier (BBB) and histopathological outcome were assessed ۲۴h after TBI. The neurological outcome score was determined using the veterinary coma scale (VCS).Results: Significant reductions in brain water content (P<۰.۰۰۱, P<۰.۰۵ and P<۰.۰۱, respectively) and BBB permeability (P<۰.۰۰۱) appeared in the treated groups with E۲, Vit D, and E۲+Vit D compared to the vehicle group. Twenty-four hours after the injury, the neurological scores in the E۲, Vit D, and E۲+Vit D groups increased significantly compared to the vehicle group (P<۰.۰۵). Dramatic improvement in histopathological outcome was also observed in the treated groups compared to the vehicle group.Conclusion: Alone and combined consumption of estrogen and vitamin D may similarly decrease the development of brain edema and improve the neurological and histopathological consequences of TBI. Therefore, consumption of vitamin D did not enhance the neuroprotective effect of estrogen in TBI.

Traumatic brain injury, Vitamin D, estrogen, Brain edema, Blood brain barrier