Ali Alirezaie Alavijeh - Clinical Department, Shahrekord University, Shahrekord, Iran
Masoomeh Dadpay - Department of Pathology, Medical Faculty, AJA University of Medical Sciences, Tehran, Iran
Mohammad Barati - Department of Applied Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran

Background: Surgical sutures are sterile filaments to support the healing process. Also Suture as a major part of surgical site management can challenge surgical wound healing. The objective of the current study was to prevent surgical site infection by creating a new antibacterial silk-nano cefixime suture.Methods: The silk suture became silk-nano cefixime suture by immersing in the ۱, ۲, and ۳ wt % cefixime solutions. The antibacterial activity of silk-nano cefixime suture was tested on E. coli (ATCC۲۵۹۲۲). The healing effect of silk-nano cefixime suture was evaluated by H&E staining sections and compared between the control and treated groups on days ۳, ۸ and ۱۴. The obtained quantitative data were presented as mean ± standard deviation. In order to compare the mean of quantitative variables between the studied groups, general linear model of statistical analysis test and ۳-D SPSS charts were used (P ≤ ۰.۰۵).Results: The inhibition zone diameter of E. coli (ATCC۲۵۹۲۲) was ۲۵ mm and no statistically significant difference was found between the healing properties (P≥.۰۵). There was a significant difference in the mean of Polymorphonuclear leukocytes (PMN) and macrophage infiltration between the control and treated groups on day ۸ (p≤ ۰.۰۵).Conclusion: Our data indicate that silk-nano cefixime suture has the potential to reduce the risk of surgical site infections. Finally, it is strongly recommended to introduce silk-nano cefixime suture as a reliable substitute for current surgical sutures.

Keyword: Silk suture, Nano-Cefixime trihydrate, Antibacterial Activity, Healing properties