Mahmood Barati - Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
Mazaher Gholipourmalekabadi - Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
Javad Ranjbari - Department of Biotechnology, Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Pegah Shakib - Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Mohammad Ali Bahar - Department of Immunology and Burn Research Center, Iran University of Medical Sciences, Tehran, Iran
Ali Samadikuchaksaraei - Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

Background: Pseudomonas aeruginosa infection is one of the major challenges in burn patients. This organism is resistant to a wide range of antibiotics. The aim of this study was to investigate the antibiotic susceptibility and genetic relatedness in Pseudomonas aeruginosa isolated from patients admitted to Motahari Burn Center, Tehran, Iran.Methods: This study was conducted on ۱۸۶ burn patients with Pseudomonas aeruginosa colonization following admission in Motahari Burn Center during one year. Antibiotic resistance test was performed by disc diffusion method and genetic relatedness was evaluated by PCR-RAPD analysis.Results: The highest and lowest percentage of resistance was observed against ceftizoxime and ciprofloxacin. The genotyping study by RAPD PCR technique revealed ۵۷ different genotypes, among which RAPD ۵, RAPD ۸, and RAPD ۹ were the most prevalent patterns and produced by ۱۴%, ۹%, and ۷% of the isolates, respectively.Conclusion: In total, no association was found between RAPD genotypes and antibiotic resistance patterns, and death rate.

RAPD PCR, Pseudomonas aeruginosa, Antibiotic Resistance, Genotypes