Polymorphism of Vascular Endothelial Growth Factor Gene−۱۱۵۴ G/A in Women with Recurrent Miscarriage and IVF Failure; A case–control study

Background: Vascular endothelial growth factor A (VEGFA) plays a key role in angiogenesis during human placenta formation and its abnormal expressions have been reported in placental tissues of women with recurrent miscarriage (RM). Objective: The aim of this study was to evaluate the correlation of polymorphism of VEGF۱۱۵۴ G/A gene in RM and In Vitro Fertilization (IVF) failures. Methods: The peripheral blood samples of women with RM, IVF failures and healthy women with live born children, as control group were collected. DNA samples were isolated and VEGF ۱۱۵۴G/A polymorphism was evaluated by polymerase chain reaction and restriction fragment length polymorphism. Single nucleotide polymorphism scanning was done using MnII restriction enzymes for ۱۱۵۴ G/A. Results: The findings of this study showed that the VEGF ۱۱۵۴ A/A and VEGF ۱۱۵۴ G/A mutation frequencies in both RM and IVF failure groups were significantly higher than those in the control group (p=۰.۰۰۵). The homozygous AA mutant genotype frequency in the control group was ۰, While in the RM and IVF failure groups it was ۳۰% and ۱۳.۹%, respectively (p=۰.۰۰۵). Moreover, the heterozygous AG genotype frequencies were higher in the RM (۶۶.۷%) and IVF failure (۷۷.۸%) compared to those in the control group (۵۸.۳%). Conclusions: It was concluded that VEGF ۱۱۵۴ A/A and VEGF ۱۱۵۴ G/A polymorphisms were associated with both RM and IVF failures. However, their relation with IVF failures was more common than RM in A/G genotype, while in A/A, the RM was higher than IVF failure.

Keywords:

Recurrent miscarriage , IVF failures , Vascular Endothelial Growth Factor , Polymorphism