Mehrnaz Karimi - Master of Science, Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Masoumeh Mirzaie - Master of Science, Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Mohammad Khaksari - Professor, Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
Mahboobeh Akbari - Master of Science, Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Mahdieh Nazari - Assistant Professor, Neuroscience Research Center, Institute of Neuropharmacology, Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Background: Chondroitin sulfate proteoglycans (CSPGs) are the major cause of axonal regeneration failure at the site of lesion in spinal cord injury (SCI). Inflammation is believed to stimulate the upregulation of CSPGs expression. Recent evidence showed that trehalose reduces the development of inflammation in SCI. The aim of this study was to investigate the effect of trehalose on neurocan and Neural-Glial Antigen ۲ (NG۲) mRNA levels in SCI in rats. Methods: In this experimental study, male rats were divided into six groups (n=۱۵). Sham (laminectomy), SCI (laminectomy and SCI), vehicle (laminectomy and SCI, treated with phosphate buffer saline), and T۱۰, T۱۰۰ and T۱۰۰۰ (laminectomy and SCI, treated with ۱۰, ۱۰۰ and ۱۰۰۰ mM trehalose). Five rats in each group were sacrificed at ۱, ۳ and ۷ days post-injury to measure neurocan and NG۲ mRNA levels in lesion. Statistical analysis was performed using Kruskal-Wallis methods followed by the Mann-Whitney test. Results: Findings indicated that SCI upregulated neurocan and NG۲ mRNA levels at all times. No significant difference was observed in neurocan and NG۲ gene transcripts between SCI and vehicle groups (p >۰.۰۵). However, ۱۰ mM trehalose downregulated the mRNA level of both neurocan (۰.۷۶ and ۰.۶۵ fold) and NG۲ (۰.۷۵ and ۰.۷۰ fold) at ۳ and ۷ days post-SCI compared to vehicle group (p p <۰.۰۱, respectively). Conclusion: Collectively, treatment with low dose trehalose showed a decrease in neurocan and NG۲ mRNA levels in spinal cord injured rats.

Spinal cord injury, Trehalose, Chondroitin sulfate proteoglycans