Enterovirus Detection in Patients Suspected Aseptic Meningitis by RT-PCR in Kermanshah, Iran

Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JKMU-25-1_003

تاریخ نمایه سازی: 19 دی 1401

Abstract:

Background: Aseptic meningitis is frequently caused by viral agents, particularly human enterovirus. Several methods of Reverse Transcription PCR(RT-PCR) have recently been introduced and modified for better diagnosis of enteroviral infection in meningitis. This study aimed to determine enteroviruses in patients with suspicion of aseptic meningitis using RT-PCR in the West of Iran. Methods: In this study, ۱۲۰ CSF samples were collected from patients hospitalized with the suspicion of aseptic meningitis in Imam Reza Hospital of Kermanshah, Iran.RT-PCR was used to diagnose enteroviruses. The cDNA recovered from RT-PCR was purified using a DNA purification kit and sequenced to confirm viral genome. Sequence data were analyzed for homology using the Gen Bank database. Results: The samples were collected from ۶۳ (۵۲.۵%) men and ۵۷ (۴۷.۵%) women with an average age of ۳۱.۵ ± ۲۹.۴ years. Of the samples tested, ۴ cases (۳.۳۳%) yielded positive results for enterovirus. The results of sequence data analysis confirmed all positive cases as Enterovirus type ۷۱. The biochemical (protein and glucose) and cytological analyses of positive CSF samples showed no significant changes. Conclusion: According to the results, Enterovirus type ۷۱is one of the common causes of enteroviral meningitis in patients in Kermanshah. The RT-PCR technique can rapidly diagnose Enterovirus type ۷۱ and help better treatment of the patients and prevent the unnecessary use of antibiotics. So, molecular methods can reduce the cost of patients’ treatments and prevent drug resistance among bacteria. It can also provide a better picture of enteroviral infection in our region.

Authors

Alisha Akya

Associate Professor of Medical Microbiology, Nosocomial Infection Research Centre, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Azam Elahi

MSc Student of Medical Microbiology, Student Research Committee, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Mohsen Moghoofei

Ph.D. Candidate, Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Roya Chegenelorestani

MSc Student of Medical Microbiology, Student Research Committee, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Ahmad Nejati

Assistant Professor, Department of Virology Faculty of public health, Tehran University of medical sciences, Tehran, Iran

Farhad Babaei

Assistant Professor, Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

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  • Dalwai A, Ahmad S, Al-Nakib W. Echo viruses are a ...
  • Rasti M, Samarbaf-Zadeh AR, Makvandi M, Shamsi-Zadeh A. Relative Frequency ...
  • Brunel D, Leveque N, Jacques J, Renois F, Motte J, ...
  • Sadeuh-Mba SA, Bessaud M, Massenet D, Joffret ML, Endegue MC, ...
  • Simmonds P, Welch J. Frequency and Dynamics of Recombination within ...
  • Dierssen U, Rehren F, Henke-Gendo C, Harste G, Heim A. ...
  • Hyeon JY, Hwang S, Kim H, Song J, Ahn J, ...
  • Tong CYW, Bible JM. Global epidemiology of enterovirus ۷۱. Future ...
  • Perez-Velez CM, Anderson MS, Robinson CC, McFarland EJ, Nix WA, ...
  • Kargar M, Sadeghipour S, Nategh R. Environmental surveillance of Non-Polio ...
  • Archimbaud C, Chambon M, Bailly JL, Petit I, Henquell C, ...
  • Allan Nix W, Steven Oberste M, Pallansch MA. Sensitive, Seminested ...
  • Martinez Planas A, Munoz Almagro C, Luaces Cubells C, Noguera ...
  • Van Loon AM, Cleator GC, Ras A. External quality assessment ...
  • Giri A, Arjyal A, Koirala S, Karkey A, Dongol S, ...
  • Meqdam MM, Khalousi MM, Al-Shurman A. Enteroviral meningitis in Northern ...
  • Racaniello VR. Picornaviridae: the viruses and their replication ۲۰۰۱ ...
  • McMinn PC. An overview of the evolution of enterovirus ۷۱ ...
  • Perera D, Podin Y, Akin W, Tan CS, Cardosa MJ. ...
  • Ho Dang Trung N, Le Thi Phuong T, Wolbers M, ...
  • Kupila L, Vuorinen T, Vainionpaa R, Hukkanen V, Marttila RJ, ...
  • Verboon-Maciolek MA, Nijhuis M, van Loon AM, van Maarssenveen N, ...
  • Mamishi S, Rahimi P, Sohrabi A, Sabuni F, Edalat R, ...
  • Roohandeh A, Rahimi P, Sohrabi A, Mobasheri M, Azadmanesh K, ...
  • Hosseininasab A, Alborzi A, Ziyaeyan M, Jamalidoust M, Moeini M, ...
  • Seo JH, Yeom JS, Youn HS, Han TH, Chung JY. ...
  • Vuorinen T, Vainionpaa R, Hyypia T. Five years' experience of ...
  • Antona D, Leveque N, Chomel JJ, Dubrou S, Levy-Bruhl D, ...
  • Rice P. Viral meningitis and encephalitis. Elsevier Ltd. ۲۰۱۳; ۴۱(۱۲):۶۷۸-۸۲ ...
  • Kim HJ, Kang B, Hwang S, Hong J, Kim K, ...
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