The Effect of Endurance Training on MiR-۱۵۵ Expression, STAT۳ Gene Expression, and Interleukin ۶ Protein in Mice with Breast Cancer

Background & Aims: Endurance training has an important role in the prevention and adjuvant therapy of breast cancer. The aim of the present study was to investigate the role of endurance training on miR- ۱۵۵ expression, signal transducer and activator of transcription-۳ (STAT۳) gene expression, and interleukin ۶ (IL-۶) protein in breast cancer tumor in mice. Methods: In this study, ۱۶ female Balb/C mice were randomly divided into exercise-tumor (ET) and rest-tumor (RT) groups. The mice were oriented in the environment and one million estrogen-dependent breast cancer cells (MC۴L۲) were injected into each mouse. Subsequently, the ET group performed endurance exercise, ۵ days per week for ۶ weeks. Tumor volume was measured by a digital caliper weekly. Finally, the mice were sacrificed and tumor tissue was removed and kept in -۷۰°C. Then, RNA was extracted by the Trizol protocol and complementary DNA (cDNA) was synthesized according to guidelines of the Kit Company. Consequently, the real-time PCR method was performed and data was collected. Results: Significant differences were observed between the ET and RT groups in the STAT۳ gene expression, miR-۱۵۵ expression, and IL-۶ protein (P < ۰.۰۵). These results were consistent with tumor growth rate. Conclusion: Exercise can reduce miR-۱۵۵ expression, STAT۳ gene expression, and IL-۶ protein in tumor tissue. Due to the reduction in miR-۱۵۵ expression, STAT۳ gene expression, and IL-۶ protein in the ET group, it can be claimed that endurance training can be used as adjuvant therapy by decreasing of oncogenic and inflammation factors.

Keywords:

miR-۱۵۵ , STAT۳ gene , Interleukin ۶ (IL-۶) , breast cancer , Endurance Training