Evaluation of the uncommon bacterial pathogens in urogenital infection of patients referred to Iran Hospital with phenotypic and molecular methods

Background and aim: One of the most common infections, especially nosocomial infections in humans, is urinary tract infection (UTI). UTI-causing agents include bacteria, fungi and viruses. Bacteria, especially Escherichia coli with about ۸۵% is the most common cause of this type of infection. Other bacteria include Klebsiella, Enterococcus, Pseudomonas, Staphylococcus, Proteus, Neisseria, Mycoplasma and Chlamydia. The aim of this study is evaluation the prevalence of uncommon bacteria in the incidence of UTI using phenotypic and molecular (PCR) methods detection. Materials and methods: In this study, during six months, ۲۳۵ urine samples were collected from patients referred to Iran Hospital who had UTI symptoms, and simultaneously with microbial culture in the laboratory, DNA extraction was performed using extraction kits and PCR test with specific primers to detect these bacteria in each Samples were taken. Results: In the samples, ۱۳۷ samples (۵۸.۲۹%) contained uncommon UTI bacteria. The prevalence of uncommon bacteria was as follows: Pseudomonas aeruginosa ۷۴ samples (۳۱.۴%), Enterococcus faecalis ۶۱ samples (۲۵.۹%), Klebsiella pneumoniae ۳۲ samples (۱۳.۶%), Staphylococcus saprophyticus ۲ Sample (۱۰.۲%), Proteus Myrabilis ۱۳ specimens (۵.۵%), Neisseria gonorrhoea ۷ specimens (۲.۹%), Chlamydia trachomatis and Mycoplasma ۵ specimens each (۲.۱%). Only ۱۹.۷% of samples containing uncommon bacteria were detected by routine laboratory methods (phenotypic diagnosis). Conclusion: The prevalence of these bacteria varies according to the geographical location, level of health, health control and level of culture of each country. Delay in accurate identification of these bacteria increases bacterial resistance to antibiotics prescribed by physicians.

Keywords:

Urinary tract infections , uncommon bacteria , molecular and phenotypical diagnosis , عفونت ادراری , باکتری های غیرشایع , تشخیص مولکولی و فنوتیپی