Thermodynamic Characterization of thionine dye with ct- DNA

The ct-DNA binding properties of thionine (Th) including binding constant and thermodynamic parameter have been systematically studied by spectrophotometric method. The binding constant and stoichiometry were determined by analysis of optical absorption spectra of the Th dye at various ct- DNA concentrations using SQUAD software. The binding of Th to ct-DNA is quite strong as indicated by remarkable hypochromicity, red shift and equilibrium binding constant (Kb). Van’t Hoff plot of 1/T versus ln Kb suggests that the Th dye binds exothermically to ct-DNA which is characterized by large negative enthalpy and entropy changes. This suggests that Th dye possibly bind to ct-DNA via electrostatic by the intercalation mode.

Keywords:

Thionine , Thermodynamic of binding , Optical absorption , SQUAD software