Study of the antimicrobial effects of condition medium from umbilical cord stem cells

Antimicrobials are generally said to the materials that are used to fight with micro-organisms to eradicate or inhibit their growth. Condition medium (condition culture medium) is secreted for proliferation and cell culture of Mesenchymal stem cells in laboratory culture medium. This compound has factors such as TGF-B, TNF, GM-SCF, KGF, PDGF, VEGF, EGF, IL-۱, IL-۶, IL-۸, BFGF, TGF-α, IGF-۱, IGF-۲, MCP-۱, HGF, Fibronectins and Collagen. Considering the presence of these factors and their role in the body, the aim of this study has been to investigate the effect of Condition Medium of Fetal umbilical cord. In this study ۵ encoded bacterial standard isolations including Escherichia Coli, Staphylococcus, Pseudomonas, Salmonella and Listeria were investigated using MBC and MIC method and a standard encoded Candida albicans yeast using Phagocytosis test in confluence of the condition Medium of fetal umbilical cord. The results of MIC and MBC showed that Condition Medium has the effect of growth inhibition in ۱۰۰% of condition medium density on Salmonella and Listeria monocytogenes but it doesn’t have any effect on growth inhibition of the rest of the bacteria. Also disc results showed the ineffectiveness the material on the growth of microorganisms except for the bacteria mentioned earlier. Phagocytosis test showed reinforcement in Phagocytosisic effect and cellular immune caused from it in adjacent samples with Condition medium cells of fetal umbilical cord. Furthermore, the macroscopic and morphologic study showed clearly that the size of Phagocytosis cells in treatment group is bigger than their size in the control group. Overall, we can say that medium condition of the stems cells of fetal umbilical cord don’t have much antibacterial and antifungal effect, and this effect was seen insignificantly in some cases such as Salmonella and Listeria monocytogenes and it can’t be a substitute for antibacterial materials.

Keywords:

Antimicrobials , Condition Medium , Stem Cells , Umbilical cord