Chemiluminescence Determination of Hydroxyzine and its Metabolite Cetirizine
عنوان مقاله: Chemiluminescence Determination of Hydroxyzine and its Metabolite Cetirizine
شناسه ملی مقاله: JR_ANALCH-3-2_010
منتشر شده در در سال 1395
شناسه ملی مقاله: JR_ANALCH-3-2_010
منتشر شده در در سال 1395
مشخصات نویسندگان مقاله:
Ali Mokhtari - Department of Chemistry, Golestan University, Gorgan, IR Iran
Mahboobeh Benam - Faculty of Chemistry, Kharazmi University, Tehran, I.R. Iran
Mohsen Keyvanfard - Department of Chemistry, Majlesi Branch, Islamic Azad University, Isfahan, I.R. Iran
Mehrgan Ghazaeian - Faculty of Chemistry, Islamic Azad University North Tehran Branch, Tehran, I.R. Iran
خلاصه مقاله:
Ali Mokhtari - Department of Chemistry, Golestan University, Gorgan, IR Iran
Mahboobeh Benam - Faculty of Chemistry, Kharazmi University, Tehran, I.R. Iran
Mohsen Keyvanfard - Department of Chemistry, Majlesi Branch, Islamic Azad University, Isfahan, I.R. Iran
Mehrgan Ghazaeian - Faculty of Chemistry, Islamic Azad University North Tehran Branch, Tehran, I.R. Iran
A chemiluminescence (CL) method has been developed for the determination of Hydroxyzine (Hyd) and its metabolite Cetirizine (Cet). The method is based on sensitizing effect of Cet and Hyd on the weak CL reaction between Ru(phen)۳۲+ and acidic Ce(IV). A mechanism for the CL reaction has been proposed on the basis of UV-Vis, fluorescent and CL spectra. By using the recommended procedure, the calibration graphs were linear over ۰.۱۴ to ۱۴.۰ μg mL-۱ (r۲=۰.۹۹۶۶) and ۰.۰۷ to ۱۰.۰ μg mL-۱ (r۲=۰.۹۹۸۴) for Hyd and Cet, respectively. The limits of detections were ۰.۰۶ μg mL-۱ and ۰.۰۳ μg mL-۱ for Hyd and Cet, respectively. The percent of relative standard deviations (n=۱۱) for ۱.۰ μg mL-۱ of Hyd and ۰.۷۴ μg mL-۱ of Cet were ۴.۵ and ۲.۳%, respectively. The broad time profile of Hyd and Cet allowed us to determine Hyd contents in plasma samples with minimum blank interferences from cysteine and ascorbic acid. The method has been satisfactorily used for the determination of Hyd and Cet in syrups, tablets and human plasma.
کلمات کلیدی: Chemiluminescence, Hydroxyzine, Cetirizine, Pharmaceuticals, Plasma
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1597535/