Parvaneh Mirabi - Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Mohamad Javad Chichi - Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
Sedighe Esmaeilzadeh - Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Seyed Reza Nabavi - Department of Applied Chemistry, University of Mazandaran, Babolsar, Iran
Seyed Gholamali Jorsaraei - Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Mahjobeh Ehsani - Faculty of Chemistry, University of Mazandaran, Babolsar, Iran

It has been documented that specific follicular fluid (FF) biochemical characteristics may be essential to determining oocyte quality. Lipid derivatives have most important role in the fertilization process and embryonic development. MALDI mass spectrometry is used for the diagnosis of biomolecules in the FF and serum of infertile women. FF and blood samples from ۱۳ infertile women (۲۰-۳۸years old) undergoing intracytoplasmic sperm injection (ICSI) was obtained, stored frozen at -۸۰º C, and later analyzed for Lipid Derivatives. Both FF and serum samples were compared and the Mfg was used as similarity index. For comparison between the FF and serum samples for one person, the Mass spectrometry (MS) intensity ratio versus molecular weight, between the FF and serum samples were calculated. Out of ۱۳ patients, three ongoing pregnancies were observed, so the percentage of pregnancy in the studied population was ۲۵%. The patients who became pregnant after micro-injection had higher unsaturated fatty acids and omega-۳ fatty acid than other samples, but Stearic and Palmitic fatty acids were not high in these patients. A number of specific fatty esters and peptide derivatives in the FF and serum samples were found that may help to improve oocyte quality.

Follicular fluid, Infertility, MALDI mass spectrometry, Lipid derivatives