Ali Lboumhamdi - BP ۵۰۹ ERRACHIDIA MOROCCO
Mohamed Znini - BP ۵۰۹ ERRACHIDIA MOROCCO
Julien Paolini - Université de Corse
Jean Costa - Université de Corse
Lhou Majidi - BP ۵۰۹ ERRACHIDIA MOROCCO

The objective of this study is to report the comparative chemical analysis of essential oil (EO) obtained by hydrodistillation (HD) and volatile fraction (VF) detected by headspace solid phase micro-extraction (HS–SPME) isolated from Pulicaria mauritanica using Gas Chromatography–Retention Indices (GC-RI) and GC–Mass Spectrometry (GC-MS) and to evaluate in vitro the antimicrobial activity of P. mauritanica EO against three bacterial strains referenced by the ATCC (American Type Culture Collection): Escherichia coli (ATCC ۲۵۹۲۲), Staphylococcus aureus (ATCC ۲۵۹۲۳) and Pseudomonas aeruginosa (ATCC ۲۷۸۵۳) and a one yeast Candida albicans using the paper disc diffusion and minimum inhibitory concentration (MIC) assays. The chromatographic profile indicates that quantitative and semi qualitative differences between the chemical compositions of both analysed samples were observed. The antimicrobial activity tested by two methods indicates that this EO was more effective against three strains tested excepted P. aeruginosa which was most resistant. Indeed, the results of the report MBC (Minimum Bactericidal Concentration)/MIC show that the EO has a bactericidal effect on S. aureus and a bacteriostatic and fungistatic effect on E. coli and C. albicans, respectively. P. mauritanica EO seems be a promising source of natural products with potential antimicrobial activity.

Pulicaria mauritanica, Essential oil, Carvotanacetone, Antimicrobial activity, Headspace solid-phase micro-extraction, Microbial strains