Mohammad Amayreh - Chemistry Department, Faculty of Science, University of Jordan, Amman, Jordan
Mohammed Hourani - Department of chemistry, Faculty of Science, University of Jordan, Amman, Jordan

A direct method for hemoglobin determination in blood was developed using iodine-coated platinum electrode. The electrochemical behavior of iron ions in hemoglobin within human red blood cells was investigated by cyclic voltammetry at iodine-coated platinum electrode. A well-defined peak assigned to oxidation of hemoglobin was observed at about ۰.۴ V. A more prominent peak for the oxidation of hemoglobin wasobserved at pH ۵.۵ in ۰.۹% NaCl solutioncompared to phosphate puffer solution of pH ۳.۵.Using hydroxylamine in the dry form with the hemoglobin in red blood cells, enhanced thepeak current for hemoglobin oxidation by ۱۴ folds. A linear relationship between the hemoglobin oxidation peak current and the concentration of hemoglobin was illustrated within the concentration range from۱.۵۳g/dl to ۹.۲g/dl with a correlation coefficient(R۲=۰.۹۹۴۹). The estimated detection limit based on S/N = ۳ ratio was ۰.۰۰۴ g/dl. The developed method was directly applied to determination of hemoglobin without pretreatment of the blood sample. The developed method passed the tests for its liability towards interferences. The developed method was validated against the regular clinical analysis for hemoglobin. Analysis of real samples by the two methods indicated the absence of systematic errors as indicated by the confidence limits at ۹۵% confidence level. Moreover, application of the null hypothesis to the results of the two methods indicated the validity of the null hypothesis(i.e., there is no significant difference between the two methods at p=۰.۰۵).

Hemoglobin analysis, Blood analysis, Iodine-coated platinum electrode, Cyclic Voltammetry