Plasmid Profile and Characterization of Escherichia coli Isolated from Diabetic Foot Ulcers in Imo State, South Eastern Nigeria
عنوان مقاله: Plasmid Profile and Characterization of Escherichia coli Isolated from Diabetic Foot Ulcers in Imo State, South Eastern Nigeria
شناسه ملی مقاله: JR_JHEHP-8-3_001
منتشر شده در در سال 1401
شناسه ملی مقاله: JR_JHEHP-8-3_001
منتشر شده در در سال 1401
مشخصات نویسندگان مقاله:
Emmanuel Onwubiko Nwankwo - Department of Microbiology, College Of Natural Sciences, Michael Okpara University of Agriculture, Umudike. P. M. B ۷۲۶۷, Umuahia, Abia State, Nigeria.
Enyinnaya Nwagbara - Department of Medical Laboratory Services, Savealife Mission Hospital, Onitsha, Anambra state, Nigeria.
Ebubechi Uloma Okey-kalu - Department of Microbiology, College Of Natural Sciences, Michael Okpara University of Agriculture, Umudike. P. M. B ۷۲۶۷, Umuahia, Abia State, Nigeria.
خلاصه مقاله:
Emmanuel Onwubiko Nwankwo - Department of Microbiology, College Of Natural Sciences, Michael Okpara University of Agriculture, Umudike. P. M. B ۷۲۶۷, Umuahia, Abia State, Nigeria.
Enyinnaya Nwagbara - Department of Medical Laboratory Services, Savealife Mission Hospital, Onitsha, Anambra state, Nigeria.
Ebubechi Uloma Okey-kalu - Department of Microbiology, College Of Natural Sciences, Michael Okpara University of Agriculture, Umudike. P. M. B ۷۲۶۷, Umuahia, Abia State, Nigeria.
Background: The study aimed at evaluating the plasmid profile and characterization of E. coli isolated from chronic diabetic foot ulcers among patients in Imo State, South East Nigeria.
Methods: A total of ۱۵۰ wound swab samples were collected and analyzed. Standard microbiological methods processed samples. Antibiogram was done by Kirby-Bauer disc diffusion method. Tests were conducted to determine biofilm potential, ESBL and AmpC production. Plasmid profiling was done by the alkaline lysis method and Gel electrophoresis.
Results: Based on the results, out of the ۱۵۰ swab samples, ۶۱(۴۰.۶%) E. coli isolates were recovered. ۵۷.۸%, ۳۲.۸%, and ۸.۲% of the E. coli isolates were positive for biofilm formation, ESBL, and AmpC production, respectively. Further, the study revealed a pattern of multiple-drug resistance of E. coli to amoxicillin/clavulanic acid (۹۶.۷%), septrin (۹۵.۱%), and ampicillin (۹۳.۴%).
Conclusion: Analysis of the multiple antibiotic resistance (MAR) index showed that the organism had MAR index values above the low-risk region (≤۰.۲) for all the tested antibiotics. Post-curring antibiogram tests revealed that nine isolates carried plasmids, suggesting that the mode of resistance may be plasmid-mediated. Findings could act as a therapeutic guide for antibiotic prescriptions in this environment.
کلمات کلیدی: Diabetic ulcers, E. coli, antibiogram, plasmid profile, Isolates, زخم دیابتی, اشرشیاکلی, آنتی بیوگرام, ویژگی پلاسمید
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1600221/