Ehsan Moghanloo - Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Elham Ghorbani - Department of Genetics Campus۲, Guilan University of Medical Science, Rasht, Iran.
Mohammad Sadegh Beikverdi - Department of Biology Islamic Azad University Central Tehran Branch, Tehran, Iran.
Parisa Badameh - Department of Microbiology, College of Basic Science and Biology,Varamin Pishva Branch, Islamic Azad University, Varamin, Iran.
Soheila Rezaei - Department of Molecular Genetic, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Ahmad Piroozmand - Autoimmune Diseases Research Center, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Shahram Teimourian - Department of Medical Genetics, Iran University of Medical Sciences, Tehran, Iran.
Minoo Shahidi - Department of Hematology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
Ahmad Khorshidi - Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.

Background: Neutrophils are the most abundant white blood cells in humans. Recently, a novel strategy called the formation neutrophil extracellular traps (NETs) was described. NETs is a new strategy for pathogen response. This study focused on whether LPS induced NETs release in vitro in the HL۶۰ cell line. Methods: In this study, the HL۶۰ cell line was used for culture and DMSO for induction and differentiation. Flow cytometry was used to evaluate CD۱۱b in the differentiated cells, and the NBT assay was used to evaluate the functionality of the differentiated HL-۶۰ cells. Neutrophil-like cells were incubated with LPS (۲۰۰ ng/ml) for ۴۵ min, followed by incubation for ۲۵ min with ۱۰۰ ng/ml Hoechst ۳۳۳۴۲. Trypan blue as vital staining was used for viability. The statistical significance of the difference between the control and treated groups was evaluated using a one-way ANOVA. Results: Our results showed that ۷۵% NETs was produced by HL-۶۰ differentiated neutrophil cells exposed to ۲۰۰ ng/ml LPS in ۴۵ minues. Conclusion: Consequently, the LPS-induced infection and lethality may occur through various mechanisms. Thus, understanding the molecular mechanisms regulating NET formation in LPS-induced neutrophil-like cells would support the development of new therapeutic methods.

Netosis, Neutrophil, HL-۶۰, Differentiation, LPS