Prevalence of Class ۱ Integron in Klebsiella Pneumoniae Isolates from Hospitals of Sanandaj, Iran

Background and Aims: Integrons as mobile genetic elements are located on the chromosome or on a plasmid in bacteria. Integrons play a main role in the dissemination of antibiotic resistance genes among different families of bacteria. The aim of this study was to identify the prevalence of class ۱ integron in Klebsiella pneumoniae isolates from hospitals of Sanandaj, Kurdistan province, Iran. Materials and Methods: Seventy Klebsiella pneumoniae isolates were collected from Hospitals of Sanandaj. Antibiotic susceptibility pattern was performed by disc diffusion method. Class ۱ integrons gene was screened by polymerase chain reaction assay. Data were analyzed by Fisher tests with STATA software program. Results: The highest and lowest rates of resistance were related to cefotaxime and imipenem, respectively. Thirteen (۱۸.۵%) out of ۷۰ Klebsiella pneumoniae isolates caring class ۱ integron gene. Out of ۲۸ multidrug resistant isolates, ۱۱ isolates were identified to be positive for the existence of class ۱ integrons. Conclusions: class ۱ integron positive isolates, compared to class ۱ integron negative isolates, reveals resistance to more antibiotics.

Keywords:

Class ۱ Integron , Klebsiella pneumoniae , PCR assay , Class ۱ Integron , Klebsiella pneumoniae , disc diffusion method , PCR assay