فرشته غلامی نژاد - Department of Nutrition, Faculty of Health, Qazvin University of Medical Science, Qazvin, Iran.
مریم جوادی - Department of Nutrition, Faculty of Health, Qazvin University of Medical Science, Qazvin, Iran.
علی اکبر کرمی - Department of Urology, Velayat Hospital, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
فاطمه علیزاده - Metabolic Diseases Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
ماریا کاویانپور - Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
حسین خادم حقیقیان - Department of Nutrition, Faculty of Health, Qazvin University of Medical Science, Qazvin, Iran.

Background and Aims: Decreased sperm motility and increased level of oxidative stress are major causes of male infertility. This study was designed to evaluate the effect of propolis supplementation on spermatogram and reproductive hormones in asthenozoospermic men. Materials and Methods: In this randomized, double-blind controlled clinical trial, ۶۰ asthenozoospermic men attending an infertility clinic in Velayat Hospital in Qazvin, Iran, were randomly assigned to one of intervention and placebo groups (n=۳۰ for each group). For ۱۰ weeks each participant in the intervention group took ۱۵۰۰ mg of propolis daily, while in the placebo group they received daily placebo. Sperm parameters, total antioxidant capacity, concentrations of malondialdehyde of plasma, inflammatory markers and reproductive hormones were measured at the baseline and at the end of the interventions. Results: Out of ۶۰ who participated in this study, ۲۹ men in the intervention group and ۲۸ men in the placebo group completed the study. After the intervention, concentration and percentage of motile sperms as well as total antioxidant capacity of plasma significantly increased while the concentration of plasma malondialdehyde and inflammatory markers significantly decreased in the intervention group compared to the placebo group (p<۰.۰۵). Conclusions: Propolis supplementation led to increase in the concentration and motility of asthenozoospermic sperms and reduction of oxidative stress and inflammatory markers. Since increase in reactive oxygen species has been observed in abnormal sperms, oral intake of propolis may be one of the ways to deal with oxidative damage in spermatozoa of infertile men.

Asthenozoospermia, Oxidative stress, Propolis, Spermatogram, Propolis, Spermatogram, Asthenozoospermia, Oxidative stress.