Shiva Aghaei - Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Seyedhossein Hekmatimoghaddam - Department of Advanced Medical Sciences and Technologies, Faculty of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ۳Department of Laboratory Sciences, Faculty of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yaz
Mehdi Kalantar - Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Mohammad Hassan Sheikha - Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Mohammad Sobhan - Department of Radiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Ali Jebali - Department of Advanced Medical Sciences and Technologies, Faculty of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ۳Department of Laboratory Sciences, Faculty of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yaz

Background and Aims: Magnetic resonance imaging (MRI) plays an essential role in molecular imaging by delivering the contrast agent into targeted cells. The aim of this study was to evaluate the use of magnetic nanoparticles containing iron oxide and silver (Fe۳O۴@Ag core-shell nanoprobe) as a contrast agent for the detection of ovarian cancer cell line ovcar-۳. Materials and Methods: Co-precipitation method was used for synthesis of Fe۳O۴Ag nanoprobe which was then characterized by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and scanning electron microscope (SEM). To evaluate the ability of this nanoprobe in detection of the ovcar-۳ cell line by MRI, the cells were exposed to different (۵ to ۵۰ µg/mL) concentrations of Fe۳O۴@Ag nanoprobe before contrast intensity calculation by MRI.  Results: SEM images revealed that Fe۳O۴@Ag nanoparticles are spherical, about ۱۰۰ nm. FTIR showed strong absorbance picks belonging to the stretching vibration of Ag and Fe-O. It was found that contrast intensity of Fe۳O۴@Ag nanoprobe decreases as concentration decreases. Statistical analysis revealed significant difference in concentrations of ۳۰, ۴۰ and ۵۰ μg/mL, compared to control (p<۰.۰۵). In the presence of Ovcar-۳ cells, higher concentrations (۱۰, ۲۰, and ۳۰ μg/mL) of nanoparticles also significantly increased contrast intensity in comparison with control (p<۰.۰۵). Conclusions: This novel magnetic nanoparticle can be used as an effective contrast agent for improving MRI in detection of ovarian cancer cells. The sensitivity of this contrast agent may be improved by binding to targeting molecules such as antibody and aptamer.

Contrast agent, Fe۳O۴@Ag nanoprobe, Magnetic resonance imaging, Ovarian cancer, ماده ایجاد کننده کانتراست تصویری, نانوپروب Fe۳O۴@Ag, سرطان تخمدان, ام آر آی