An in vitro investigation of the apoptosis-inducing activity of corosolic acid in breast cancer cells

Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJBMS-26-4_011

تاریخ نمایه سازی: 9 اسفند 1401

Abstract:

Objective(s): Breast cancer is the most prevalent cancer among females with different molecular subtypes. Corosolic acid is a pentacyclic triterpenoid with anti-cancer properties. Materials and Methods: The MTT assay was used to assess the cytotoxic activity of corosolic acid on MDA-MB-۲۳۱ and MCF۷ cell lines. To determine the apoptotic cells, the flow cytometry technique was utilized. The expression levels of apoptosis-related genes and proteins were quantified using quantitative real time-PCR (qRT-PCR) and Western blotting methods. The activity of caspase enzymes was measured by spectrophotometry.  Results: Corosolic acid significantly inhibited the proliferation of both cell lines compared with controls. This agent markedly induced apoptosis in MDA-MB-۲۳۱ cells but did not affect MCF۷ cells compared with controls. Treating the MADA-MB-۲۳۱ and MCF۷ cell lines with corosolic acid showed an inducing effect on apoptosis-associated caspases, including Caspase-۸, ۹, and -۳, in MADA-MB-۲۳۱ cells with no effect on apoptotic markers in MCF۷ cells. Further experiments uncovered corosolic acid-induced apoptosis in MADA-MB-۲۳۱ cells by decreasing the expression of the phosphorylated form of JAK۲ and STAT۳ proteins.Conclusion: The present data suggested that corosolic acid is an apoptosis-inducing phytochemical in triple-negative breast cancer MADA-MB-۲۳۱ cells. Also, corosolic acid triggered apoptosis in these cells by stimulating both pathways of apoptosis and inhibiting the JAK/STAT signaling. Furthermore, corosolic acid was found to inhibit MCF۷ cell proliferation by a non-apoptotic mechanism.

Authors

Saade Abdalkareem Jasim

Medical Laboratory Techniques Department, Al-Maarif University College, Al-anbar-Ramadi, Iraq

Omer Zedan Khalaf

Al-Anbar Health Directorate, Iraq

Shadia Hamoud Alshahrani

Medical Surgical Nursing Department, King Khalid University, Almahala, Abha, Saudi Arabia

Kadda Hachem

Laboratory of Biotoxicology, Pharmacognosy and Biological Valorization of Plants (LBPVBP), Faculty of Sciences, University of Saida-Dr Moulay Tahar, ۲۰۰۰۰ Saida, Algeria

Shukhrat Ziyadullaev

Department of Internal Diseases, Vice-rector for Scientific Affairs and Innovations, Samarkand State Medical University, Amir Temur Street ۱۸, Samarkand, Uzbekistan

Abduladheem Turki Jalil

Medical Laboratories Techniques Department, Al-Mustaqbal University College, Babylon, Hilla, ۵۱۰۰۱, Iraq

ChangMing Wang

International College, Krirk University, Bangkok, ۳ Ram Inthra Rd, Khwaeng Anusawari, Khet Bang Khen, Krung Thep Maha Nakhon, ۱۰۲۲۰, Thailand

Rahman S. Zabibah

Medical Laboratory Technology Department, College of Medical Technology, Islamic University, Najaf, Iraq

Yousef A. Bin Jardan

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia

Qutaiba A. Qasim

College of Pharmacy, Al-Ayen University, Thi-Qar, Iraq

Marwah Suliman Maashi

Medical Laboratory Science Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah ۲۱۵۸۹, Saudi Arabia

Yasser Fakri Mustafa

Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul-۴۱۰۰۱, Iraq

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