S.B.M. Sembiring - National Research and Innovation Agency, Indonesia
N.A. Giri - Research Center for Fishery, National Research and Innovation Agency, Indonesia.
R. Pratiwi - Gajah Mada University, Indonesia
H. Haryanti - Research Center for Fishery, National Research and Innovation Agency, Indonesia.
S. Hadisusanto - Gadjah Mada University, Indonesia

The experiment was carried out for ۱۲۰ days to determine the optimal dietary protein requirement for the growth of sandfish, Holothuria scabra. The experiment used ۱۲ plastic containers sized ۵۹×۴۷×۳۷ cm, and each container stocked ۳۰ individual hatchery-produced sandfish juveniles (initial weight= ۲.۷۰±۰.۰۱ g). Experimental diets with different levels of protein, i.e., ۱۰% (A), ۲۰% (B), and ۳۰% (C) as a treatment; it was as dry pellet with ۳ mm diameters.  Sandfish were fed with experimental diets once in the afternoon at ۲ % biomass. Results of the experiment showed that sandfish fed a ۲۰% protein diet has the highest final weight (۵.۱۰±۰.۰۲ g), specific growth rate (SGR; ۰.۵۳±۰.۰۱ % day-۱), and feed efficieny (FE; ۶۲.۴۸±۰.۱۳) and is significantly different compared to other treatments (p<۰.۰۵). On the other hand, feeding with different dietary protein levels did not influence the survival rate greatly (p>۰.۰۵), ranging from ۸۵.۰۰-۹۵.۸۳%. The total amino acid content of sandfish juveniles in treatment B was highest (۶.۶۲%) and followed by C (۶.۲۷%) and A (۴.۷۵%). Likewise, the highest fatty acid content was also found in treatment B (۰.۴۴۰%) followed by treatments A and C of ۰.۴۱۶%; ۰.۳۳۲% respectively.  Results of the present study found the best feed was treatment B at ۲۰ % protein content. Moreover, based on statistical analyses, it is suggested that dietary protein requirement for maximum growth of juvenile sandfish is ۱۶%.

Amino acid, Fatty acid, Growth, Protein level, Sandfish