Mapping QTLs for Agronomic Traits in Rice Under Water Stress Condition Using Iranian Recombinant Inbred Lines Population

In the current study, a set of ۹۶ recombinant inbred lines (RIL) at F۸, derived from a cross between two varieties, Anbarbu (sensitive to drought stress) and Sepidroud (tolerant to drought stress) were used. The experiment was performed at Gonbad Kavous located in the Golestan province of Iran in ۲۰۱۰–۲۰۱۱ using two augmented designs at normal and stress conditions, separately. The RIL population was assessed for ۱۱ agronomic traits including grain yield, panicle number, panicle length, grain number, grain weight, straw weight and secondary branches number. Linkage map with ۱۳۵ microsatellite markers was used to map quantitative trait loci (QTLs) for agronomic traits under normal and drought stress conditions. Using the composite interval mapping, a total of ۱۶ QTLs were identified for the studied traits in both conditions. We detected ۸ QTLs located on chromosomes ۱, ۳, ۴, ۸ and ۹ in the non–stress condition. Favorable alleles were contributed by Spidroud for straw weight and grain number. Under drought condition, several QTLs were identified which were located on chromosomes ۱, ۲, ۴, ۵, ۶, ۹ and ۱۰. Among the detected QTLs, ۶ explained more than ۲۰% of the phenotype variation.

Keywords:

Drought , QTL mapping , Rice