Myoglobin Detection Using Disposable Electrochemical Immuno-sensor

Publish Year: 1387
نوع سند: مقاله کنفرانسی
زبان: English
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ICNN02_073

تاریخ نمایه سازی: 27 شهریور 1391

Abstract:

The unprecedented interest in the development and exploitation of analytical devices for detection,quantification and monitoring of specific chemical species has led to the emergence of biosensors. The estimation of metabolites such as glucose, urea, cholesterol and lactate in whole blood is of central importance in clinical diagnostics. Biosensors represent a new trend emerging in the diagnostic technology[2]. Although a diverse range of sensor devices have been developed very few enter commercial production due to issues such as cost and/or incompatible with mass manufacture. The main objective of the study was to develop disposable electrochemical immuno-sensors based on polymer electrodes. Unlike traditional electrode materials such as platinum, gold or carbons, polymer electrodes are inexpensive (<5 cents per unit) and compatible with mass manufacture. In the following we report on the fabrication of electrochemical immuno-sensors based on conducting polymer electrodes. Conducting polymers are semi-conductor materials that are responsive to external stimuli such as antigen binding to immobilized antibody. In typical sensor applications, conducting polymers are electropolymerized onto electrode surfaces such as Pt, Au. In commercial terms, the electropolymerization process and electrode fabrication add significantly to the final cost of the sensor. Therefore, in the current study we developed a process for fabricating polymer electrodes from polypyrrole. In addition to being conductive, conducting polymers carries a cationic charge and strong binds proteins albeit non-specifically. However, selectivity in the current sensor was imparted by reacting the bound protein antigen with antibodies immobilized on paramagnetic beads (Dynabeads)

Authors

Mir-Hassan moosavy

Department of pathobiology, University of Tabriz, Tabriz, Iran

Keith Warriner

Department of Food Science, University of Guelph, Guelph, Ontario N۱G۲W۱, Canada

Subrayal Reddy

School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey, UK