Optimization of Nano-Hydrogel prepared by ionotropic gelation method using a Factorial Design study

Preparation of nanoparticle is a well-known method used to modify/control the drug release. In this study, chitosan, the deacetylated derivative of chitin, was used as a natural polymer with approved pharmaceutical applications [1] due to its biodegradability and biocompatibility [2], and low toxicity [3]. The preparation of chitosan-heparin nanoparticles was accomplished using the ionotropic gelation method in order to improvement of heparin efficacy. This technique was selected due to its simplicity and production of nanoparticles with relatively high drug loading. Heparin was selected as a model drug as its short biological half life and side effects makes it a good candidate for sustained release nanoparticulate preparation