Investigating experimental Pathogenicity of white spot syndrome virusin Litopenaeus vannamei using PCR method

Background and objective : Providing healthy food and required for increasingpopulation of the country , that their health is important for future physical andmental health , Including responsibilities that it should be considered by wisestrategy . Among these , marines have multiple capabilities for preventing diseasesand providing health and in comparison with other proteins are more completelynutrients . In this study pathogenicity of virus of white spot disease that is thecause of the death and destruction of shrimps , in the Litopenaeus vannamei havebeen studied experimentally .Materials and methods :In this research , ۲۰۰ Litopenaeus vannamei weighing approximately ۱۰-۱۲ grwere randomly selected ,and Infected animals being tested , and by usingdiagnostic kits IQ۲۰۰۰ PCR have been confirmed . From the frozen body of Indianwhite shrimps that previously have shown symptoms of white spot disease , withusing Nested Polymerase chain reaction (Nested-PCR) method , the virus existencewas proven , that was used as virus source , and during a day in several mealsalong with food was given to Litopenaeus vannamei . After ۳ days , clinicalsymptoms including : rapid separation of the cuticle from the epidermis , emptystomach and intestines , yellow and brittle of hepatopancreas , and red movinglimbs was observed . Samples of tissues from treatment groups for study PCR andnext steps were selected .Results :samples with Nested-PCR method , according to instructions of diagnostic specialkit of white spot disease , multiple reaction steps passed and Finally, by ultravioletrays were observed and recorded, of which ۹۰ samples ,in ۶۲ cases positivereactions were observed .

Keywords:

Experimental Pathogenicity , White spotsyndrome virus , Litopenaeus vannamei , PCR .