M. Mohammadian - Research Laboratory of Spectrometry & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, Tehran, ۱۶۸۴۶-۱۳۱۱۴, Iran
K Dashtian - Research Laboratory of Spectrometry & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, Tehran, ۱۶۸۴۶-۱۳۱۱۴, Iran
R Zare Dorabei - Research Laboratory of Spectrometry & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, Tehran, ۱۶۸۴۶-۱۳۱۱۴, Iran
R Sedghi - Research Laboratory of Spectrometry & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, Tehran, ۱۶۸۴۶-۱۳۱۱۴, Iran

Human respiration is accompanied by abundant physiological and pathological information, such as the change in creatinine content, which is related to chronic kidney disease (CKD); hence, monitoring breathing behavior helps in health assessment and illness prediction. Herein, we developed an electrochemiluminescence (ECL) assay based on electrodeposition of Co۳O۴/prussian blue analog thin film nanozyme on nickel mesh for the effective luminol oxidation leading to a fluorogenic reaction and subsequently highly sensitive for highly sensitive chronic kidney disease biomarker creatinine detection. Results confirm the large specific surface area and redox cation structure of as-prepared nanozyme as a synergic advantage of reasonable nanostructures for ensuring excellent mimicking-enzyme catalytic activity. The morphological and structural analyses were also confirmed by microscopic and spectroscopic analyses. This ECL platform revealed the sensitive detection of creatinine acid as a neurotransmitter in the linear range of ۰.۹ μM to ۵۰۰ μM relative to creatinine with a detection limit of ۹.۰ nM. The findings confirmed the biocompatibility and feasibility of the proposed ECL nanonzymatic strategy for detecting creatinine in biological samples as well as revealed satisfied selectivity in the presence of other biological molecules. As a proof of concept, this work sheds light on the superiority of thin film prussian blue analog to construct an robust and promising ECL strategy with low triggering potential.

Creatinine; chronic kidney disease biomarker; electrochemiluminescence; fluorometric platform; prussian blue analog thin film