Mohammad Hossein Zorman - Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad KavousUniversity, Gonbad Kavous, Golestan, Iran
Hosna Gholipour Kanani, - Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad KavousUniversity, Gonbad Kavous, Golestan, Iran
Mohammad Farhangi - Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad KavousUniversity, Gonbad Kavous, Golestan, Iran
Hosein Adineh - Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad KavousUniversity, Gonbad Kavous, Golestan, Iran

Many Nano emulsions have proved effective in anesthetization of fish. In this study, we selected ۳ anaesthetics, Nano emulsions of clove oil (NLC), Nano emulsions lemonbeeprush-clove oil (NLBC) and Tricaine Methane sulfonate (MS), to test the efficacy in anesthetization of rainbow trout Oncorhynchus mykiss. The experiments were done by ۱۲۰ fish with average weight ۵۰ ± ۱۲ g. The fish were divided into ۳ treatments (each consisting of ۱۰ fish). ۳ treatments with ۳ replicates were exposed to NBLC and NCL with ۱۰ mg L-۱ concentrations and MS with ۱۰ mg L-۱ by immersion method. The time of each sedation anaesthetics and recovery stages were ۲۴ hours. Our experiment results show that NBL (lemonbeeprush + Clove Nano Essentials Oil) can significantly increase oxygen and reduce ammonia concentration compared to the control (p< ۰.۰۵). Some metabolic factors (urea-sodium-potassium) were significantly between different experimental and control, as their highest was obtained in control. The result of this experiment clearly indicated that, NCL had a less time of sedation anaesthetics to others, without any mortality. We revealed, a briefly compares of Nano emulsions to MS in this study. (Abstracts should be ۱۰۰-۲۵۰ words in length).

Nano emulsions, metabolic factors, rainbow trout (Oncorhynchus mykiss)