Iman Maksum - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia
Firdausi Permata Latifah - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia
Ahmad Nabiel - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia
Sriwidodo Sriwidodo - Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia
Niky Lestari - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia
Mamlikatu Azizah - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia
Diandra Utami - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia

Escherichia coli is one of the most commonly used organisms for producing recombinant protein. The recognized cell genome and well-known cell factory of the bacteria make it an ideal heterologous system of preference for the production of recombinant proteins. Over recent years, this cell system has been modified to improve the production of therapeutic proteins, and several methodologies are now available. One of the scientists' most preferred strategies is maintaining cell survival by optimizing the culture conditions. This review summarises some experiments related to those culture conditions and discusses how they affect recombinant protein expression in E. coli.

Recombinant protein, Escherichia coli, Culture Expression Parameters