Hoda Shabestarian - Department of Biochemistry, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran
Masoud Homayouni Tabrizi - Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Monireh Movahedi - Department of Biochemistry, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran
Ali Neamati - Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Fariba Sharifnia - Department of Biology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran

Objective(s): The study aimed to synthesize both silver- and zinc-oxide nanoparticles utilizing the Peganum harmala smoke extract (PHSE) bio-platform to evaluate their cytotoxicity on different types of human cancer cell lines and study their anti-oxidant, and anti-angiogenic potentials. Materials and Methods: The Silver (Ag) and zinc oxide (ZnO) nanoparticles (NP) were produced utilizing the green-synthesize method by applying the PHSE bio-platform. After characterization by X-Ray Diffraction (XRD), dynamic light scattering (DLS), Field emission scanning electron microscopy (FESEM), and Fourier-transform infrared spectroscopy (FTIR) methods. MTT assay was used for the evaluation toxicity of nanoparticles. ABTS, DPPH, FRAP, and ROS for anti-oxidant capacity, chicks’ chorioallantoic membrane (CAM), and qPCR for anti-angiogenesis effects of nanoparticles were used. Results: Ag-NPs (۸۲.۴۲ nm) and ZnO-NPs (۱۶۳.۰۵ nm) inhibited prostate, ovarian, and liver malignant cells. Inhibition of ABTS•+ and DPPH•+ and increasing the rate of intracellular ROS exhibited the anti and pro-oxidant capacity of Ag and ZnO-NPs out and inside of malignant cells. Also, their anti-angiogenesis impact was verified by significant dose-dependent VEGF and VEGFR down-regulation and the decreased blood vessels in the CAM. Conclusion: The anti-oxidant, cytotoxicity and anti-angiogenesis effects of Ag and ZnO-NPs synthesized from Pecan smoke extract make it possible to use these nanoparticles in cancer chemotherapy.

Angiogenesis inhibitors, Anti-Oxidants, Metal Nanoparticles