Andi Yasmon - Department of Microbiology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital. Jl.Pegangsaan Timur ۱۶, Cikini, Jakarta Pusat ۱۰۳۲۰, Jakarta,Indonesia
Paulina Rosa Evriarti - Biomedical Science Graduate Program, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No.۵, Jakarta Pusat ۱۰۳۲۰, Jakarta, Indonesia
Yeva Rosanna - Department of Microbiology, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital. Jl.Pegangsaan Timur ۱۶, Cikini, Jakarta Pusat ۱۰۳۲۰, Jakarta, Indonesia

Background and Aim: In Indonesia, diphtheria cases caused by Corynebacterium diphtheriae are still occurring. One of the causes is probably the diphtheria toxin repressor (dtxR) gene, which influences toxin expression. In this study, the gene was characterized to determine the mutations that affect the DtxR protein. Materials and Methods: The dtxR genes of ۱۰ C. diphtheria strains isolated in Jakarta were amplified by conventional PCR. The PCR products were directed to DNA sequencing by using overlapping primers. Results and Conclusion: No mutations showed amino acid changes. We propose that the DtxR protein is conserved among bacterial strains isolated from Jakarta.

Corynebacterium diphtheriae, dtxR, Mutation, Phylogeny tree, Corynebacterium diphtheriae, dtxR, جهش, درخت فیلوژنی